Characterization of different DLC and DLN electrodes for biosensor design

R. Maalouf (1) , N. Jaffrezic-Renault (1) , O. Vittori (2) , M. Sigaud (2) , Y. Saikali (3) , H. Chebib (3) , A.-S. Loir (4) , Florence Garrelie (4) , C. Donnet (4) , T. Takeno (5) , T. Takagi (5)

1 CEGELY - Centre de génie électrique de Lyon
2 Laboratoire d'électrochimie analytique
3 Laboratory of Chemistry
4 LHC - Laboratoire Hubert Curien
5 Institute of Fluid Science

Diamond-Like Carbon and Carbon-Like Nanocomposite electrodes, novel materials in the field of biosensors, made with different ratio of sp3/sp2 carbon hybridization or doped with elements such as Ni, Si and W, were characterized electrochemically by cyclic voltammetry and by amperometric measurements towards hydrogen peroxide. SiCAr1 and SiCNi5% were chosen as sensitive transducers for elaboration of amperometric glucose biosensors. Immobilization of glucose oxidase was carried out by cross-linking with glutareldehyde. Measurements were made at a fixed potential + 1.0 V in 40 mM phosphate buffer pH 7.4. SiCAr1 seems to be more sensitive for glucose (0.6875 µA/mM) then SiCNi5% (0.3654 µA/mM). Detections limits were respectively 20 µM and 30 µM. Michaelis-Menten constants for the two electrodes were found around 3 mM. 48% and 79% of the original response for 0.5 mM glucose remained respectively for both electrodes after 10 days.

Domaines: Science des matériaux [cond-mat.mtrl-sci]

R. Maalouf, N. Jaffrezic-Renault, O. Vittori, M. Sigaud, Y. Saikali, et al.. Characterization of different DLC and DLN electrodes for biosensor design. Journal of Advanced Science, 2006, 18, 18, pp.31-36. ⟨ujm-00109718⟩