Characterization of different diamond-like carbon electrodes for biosensor design

Abstract : Diamond-like carbon (DLC) films are gaining big interest in electrochemistry research area. DLC electrodes made with different ratio of sp3/sp2 carbon hybridization or doped with different percentages of nickel were characterized electrochemically by cyclic voltammetry and by amperometric measurements towards hydrogen peroxide. SiCAr1 and SiCNi5% were chosen as sensitive transducers for the elaboration of amperometric glucose biosensors. Immobilization of glucose oxidase was carried out by cross-linking with glutaraldehyde. Measurements were made at a fixed potential + 1.0V in 40mM phosphate buffer pH 7.4. SiCAr1 seems to be more sensitive for glucose, 0.6875 µA/mM, than SiCNi5%, 0.3654 µA/mM. Detections limits were 20 µM and 30µM, respectively. Apparent Michaelis-Menten constants were found around 3 mM. Forty-eight percent and 79% of the original response for 0.5mM glucose remained after 10 days for both biosensors, respectively.
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https://hal-ujm.archives-ouvertes.fr/ujm-00140042
Contributor : Florence Garrelie <>
Submitted on : Wednesday, April 4, 2007 - 3:52:28 PM
Last modification on : Tuesday, November 19, 2019 - 2:39:30 AM

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  • HAL Id : ujm-00140042, version 1

Citation

R. Maalouf, H. Chebib, Y. Saikali, O. Vittori, M. Sigaud, et al.. Characterization of different diamond-like carbon electrodes for biosensor design. Talanta, Elsevier, 2007, 72, pp.310-314. ⟨ujm-00140042⟩

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