Delivery of molecules into corneal endothelium using nanoparticles activated by femtosecond laser pulses: proof of concept

Clothile Jumelle; Nelly Campolmi; Aurélien Bernard; Simone Piselli; Éric Audouard; Julien Granier; Hervé Soder; Philippe Gain; Gilles Thuret

Delivery of molecules into corneal endothelium using nanoparticles activated by femtosecond laser pulses: proof of concept

Clothile Jumelle ¹ Nelly Campolmi ¹ Aurélien Bernard ¹ Simone Piselli ¹ Éric Audouard ² Julien Granier ³ Hervé Soder ³ Philippe Gain ¹ Gilles Thuret ¹

1 EA 2521, JE2521, IFR143 - Biologie, Ingénierie et Imagerie de la Greffe de Cornée

2 LHC - Laboratoire Hubert Curien [Saint Etienne]

3 IMPULSION

Abstract : Purpose NanoFemtoTransfection (NFT) is an innovative and promising non-viral technique to transfer molecules into cells (Chakravarty. Nature Nanotechnology 2010;5:607). It consists in temporarily permeabilizing cell membrane by a photoacoustic effect obtained by nanoparticles of black carbon activated by Ti-Saphir femtosecond laser (fsL) pulses. Calcein (622 Da), tagged bovine serum albumine (70 kDa) and one eGFP plasmid (5 MDa) were transfected into two non-adherent cell lines (DU145 prostate-cancer and GS-9L rat gliosarcoma). Our aim was to adapt the NFT to adherent human corneal endothelial cells (HCEC)

Document type :

Conference papers

Domain :

Engineering Sciences [physics] / Optics / Photonic

Complete list of metadatas

https://hal-ujm.archives-ouvertes.fr/ujm-00757711
Contributor : Cyril Mauclair <>
Submitted on : Tuesday, November 27, 2012 - 2:38:20 PM
Last modification on : Wednesday, July 25, 2018 - 2:05:31 PM

Delivery of molecules into corneal endothelium using nanoparticles activated by femtosecond laser pulses: proof of concept

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Delivery of molecules into corneal endothelium using nanoparticles activated by femtosecond laser pulses: proof of concept

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