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Delivery of molecules into corneal endothelium using nanoparticles activated by femtosecond laser pulses: proof of concept

Clothile Jumelle 1 Nelly Campolmi 1 Aurélien Bernard 1 Simone Piselli 1 Éric Audouard 2 Julien Granier 3 Hervé Soder 3 Philippe Gain 1 Gilles Thuret 1
1 EA 2521, JE2521, IFR143 - Biologie, Ingénierie et Imagerie de la Greffe de Cornée
2 LHC - Laboratoire Hubert Curien [Saint Etienne]
3 IMPULSION
Abstract : Purpose NanoFemtoTransfection (NFT) is an innovative and promising non-viral technique to transfer molecules into cells (Chakravarty. Nature Nanotechnology 2010;5:607). It consists in temporarily permeabilizing cell membrane by a photoacoustic effect obtained by nanoparticles of black carbon activated by Ti-Saphir femtosecond laser (fsL) pulses. Calcein (622 Da), tagged bovine serum albumine (70 kDa) and one eGFP plasmid (5 MDa) were transfected into two non-adherent cell lines (DU145 prostate-cancer and GS-9L rat gliosarcoma). Our aim was to adapt the NFT to adherent human corneal endothelial cells (HCEC)
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Conference papers
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https://hal-ujm.archives-ouvertes.fr/ujm-00757711
Contributor : Cyril Mauclair <>
Submitted on : Tuesday, November 27, 2012 - 2:38:20 PM
Last modification on : Wednesday, May 27, 2020 - 9:42:02 PM

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UNIV-ST-ETIENNE | CNRS | LAHC | IOGS | PARISTECH | UDL

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Clothile Jumelle, Nelly Campolmi, Aurélien Bernard, Simone Piselli, Éric Audouard, et al.. Delivery of molecules into corneal endothelium using nanoparticles activated by femtosecond laser pulses: proof of concept. European Association for Vision and Eye Research, Sep 2012, Nice, France. pp.3432, ⟨10.1111/j.1755-3768.2012.3432.x⟩. ⟨ujm-00757711⟩

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