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Conference Papers Acta Ophthalmologica Year : 2012

Delivery of molecules into corneal endothelium using nanoparticles activated by femtosecond laser pulses: proof of concept

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Abstract

Purpose NanoFemtoTransfection (NFT) is an innovative and promising non-viral technique to transfer molecules into cells (Chakravarty. Nature Nanotechnology 2010;5:607). It consists in temporarily permeabilizing cell membrane by a photoacoustic effect obtained by nanoparticles of black carbon activated by Ti-Saphir femtosecond laser (fsL) pulses. Calcein (622 Da), tagged bovine serum albumine (70 kDa) and one eGFP plasmid (5 MDa) were transfected into two non-adherent cell lines (DU145 prostate-cancer and GS-9L rat gliosarcoma). Our aim was to adapt the NFT to adherent human corneal endothelial cells (HCEC)
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Dates and versions

ujm-00757711 , version 1 (27-11-2012)

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Clothile Jumelle, Nelly Campolmi, Aurélien Bernard, Simone Piselli, Éric Audouard, et al.. Delivery of molecules into corneal endothelium using nanoparticles activated by femtosecond laser pulses: proof of concept. European Association for Vision and Eye Research, Sep 2012, Nice, France. pp.3432, ⟨10.1111/j.1755-3768.2012.3432.x⟩. ⟨ujm-00757711⟩
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