Delivery of molecules into corneal endothelium using nanoparticles activated by femtosecond laser pulses: proof of concept

Clothile Jumelle (1) , Nelly Campolmi (1) , Aurélien Bernard (1) , Simone Piselli (1) , Éric Audouard (2) , Julien Granier (3) , Hervé Soder (3) , Philippe Gain (1) , Gilles Thuret (1)

1 EA 2521, JE2521, IFR143 - Biologie, Ingénierie et Imagerie de la Greffe de Cornée
2 LHC - Laboratoire Hubert Curien [Saint Etienne]
3 IMPULSION

Purpose NanoFemtoTransfection (NFT) is an innovative and promising non-viral technique to transfer molecules into cells (Chakravarty. Nature Nanotechnology 2010;5:607). It consists in temporarily permeabilizing cell membrane by a photoacoustic effect obtained by nanoparticles of black carbon activated by Ti-Saphir femtosecond laser (fsL) pulses. Calcein (622 Da), tagged bovine serum albumine (70 kDa) and one eGFP plasmid (5 MDa) were transfected into two non-adherent cell lines (DU145 prostate-cancer and GS-9L rat gliosarcoma). Our aim was to adapt the NFT to adherent human corneal endothelial cells (HCEC)

Domains: Engineering Sciences [physics]; Optics / Photonic

Clothile Jumelle, Nelly Campolmi, Aurélien Bernard, Simone Piselli, Éric Audouard, et al.. Delivery of molecules into corneal endothelium using nanoparticles activated by femtosecond laser pulses: proof of concept. European Association for Vision and Eye Research, Sep 2012, Nice, France. pp.3432, ⟨10.1111/j.1755-3768.2012.3432.x⟩. ⟨ujm-00757711⟩