Anticancer drug detection using a highly sensitive molecularly imprinted electrochemical sensor based on an electropolymerized microporous metalorganic framework

Anca Florea 1, 2 Zhenzhong Guo C. Cristea 1 François Bessueille 3 Francis Vocanson 4 F. Goutaland 4 S. Dzyadevych 5 R. Sandulescu 1, * N. Jaffrezic-Renault 2, *
* Corresponding author
1 Université Cluj-Napoca
2 Interfaces & biosensors - Interfaces & biocapteurs
ISA - Institut des Sciences Analytiques
3 Surfaces
ISA - Institut des Sciences Analytiques
4 LHC - Laboratoire Hubert Curien [Saint Etienne]
5 Institute of Molecular Biology and Genetics
Abstract : A simple and highly sensitive approach for the detection of the anti-neoplastic drug gemcitabine is presented, based on a one-step electropolymerized molecularly imprinted microporous-metal-organic-framework. The sensitive layer was prepared by electropolymerization of the aniline moieties of p-aminothiophenol- gold nanoparticles on the surface of gold electrodes tethered with p-aminothiophenol, in the presence of gemcitabine as a template molecule. Experimental parameters that control the performance of the sensor were investigated and optimized. Under optimal conditions a calibration curve was obtained in the linear range from 3.8 fM to 38 nM with a limit of detection of 3 fM. The obtained imprinted sensor has the advantages of easy manufacture, high sensitivity and selectivity and good reproducibility. Furthermore the feasibility of the proposed technique has been investigated on spiked serum samples and infusion solution containing gemcitabine.
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Journal articles
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https://hal-ujm.archives-ouvertes.fr/ujm-01121984
Contributor : Francis Vocanson <>
Submitted on : Tuesday, March 3, 2015 - 8:15:47 AM
Last modification on : Friday, June 21, 2019 - 7:56:04 AM

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UNIV-ST-ETIENNE | ENS-LYON | ISA | LAHC | INC-CNRS | UNIV-LYON1 | IOGS | PARISTECH | UNIV-PARIS-SACLAY

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Anca Florea, Zhenzhong Guo, C. Cristea, François Bessueille, Francis Vocanson, et al.. Anticancer drug detection using a highly sensitive molecularly imprinted electrochemical sensor based on an electropolymerized microporous metalorganic framework. Talanta, Elsevier, 2015, 138, pp.71-76. ⟨10.1016/j.talanta.2015.01.013.⟩. ⟨ujm-01121984⟩

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