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Automatic numerical focus plane estimation in digital holographic microscopy using calibration beads

Abstract : We present a new method to achieve autofocus in digital holographic microscopy. The method is based on inserting calibrated objects into a sample placed on a slide. Reconstructing a hologram using the inverse problems approach makes it possible to precisely locate and measure the inserted objects and thereby derive the slide plane location. Numerical focusing can then be performed in a plane at any chosen distance from the slide plane of the sample in a reproducible manner and independently of the diversity of the objects in the sample.
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https://hal-ujm.archives-ouvertes.fr/ujm-03571296
Contributor : Corinne Fournier Connect in order to contact the contributor
Submitted on : Sunday, February 13, 2022 - 11:31:16 PM
Last modification on : Tuesday, February 22, 2022 - 2:21:58 PM
Long-term archiving on: : Saturday, May 14, 2022 - 6:24:05 PM

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Dylan Brault, Corinne Fournier, Thomas Olivier, Nicolas Faure, Sophie Dixneuf, et al.. Automatic numerical focus plane estimation in digital holographic microscopy using calibration beads. Applied optics, Optical Society of America, 2022, 61 (5), pp.B345. ⟨10.1364/AO.444996⟩. ⟨ujm-03571296⟩

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