Automatic numerical focus plane estimation in digital holographic microscopy using calibration beads - Archive ouverte HAL Access content directly
Journal Articles Applied optics Year : 2022

Automatic numerical focus plane estimation in digital holographic microscopy using calibration beads

(1) , (1) , (1) , , (2) , (1) , (3, 4) , (1)
1
2
3
4

Abstract

We present a new method to achieve autofocus in digital holographic microscopy. The method is based on inserting calibrated objects into a sample placed on a slide. Reconstructing a hologram using the inverse problems approach makes it possible to precisely locate and measure the inserted objects and thereby derive the slide plane location. Numerical focusing can then be performed in a plane at any chosen distance from the slide plane of the sample in a reproducible manner and independently of the diversity of the objects in the sample.
Fichier principal
Vignette du fichier
2022_AO_Brault_Fournier_Autofocus_DH.pdf (3.71 Mo) Télécharger le fichier
Origin : Files produced by the author(s)

Dates and versions

ujm-03571296 , version 1 (13-02-2022)

Identifiers

Cite

Dylan Brault, Corinne Fournier, Thomas Olivier, Nicolas Faure, Sophie Dixneuf, et al.. Automatic numerical focus plane estimation in digital holographic microscopy using calibration beads. Applied optics, 2022, 61 (5), pp.B345. ⟨10.1364/AO.444996⟩. ⟨ujm-03571296⟩
54 View
56 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More