A sedimented sample of a 59 kDa dodecameric helicase yields high-resolution solid-state NMR spectra.
Abstract
Crystal clear: Preparing solid-state NMR samples that yield high-resolution spectra displaying high sensitivity is time-consuming and complicated. A sample of the 59 kDa protein DnaB, prepared simply by preparative centrifugation, provides spectra that are as good as the ones from carefully grown microcrystals.
Domains
Life Sciences [q-bio]
Origin : Files produced by the author(s)